Doubled haploid production in crop plants: a manual

1. Production of doubled haploids in crop plants. An introduction / K. J. Kasha and M. Maluszynski

2. Protocols for major crops

2.1. Doubled haploid production in barley using the Hordeum bulbosum (L.) technique / P. Hayes, A. Corey and J. DeNoma

2.2. Hordeum bulbosum (L.) Method / P. Devaux

2.3. Anther culture in barley / C. Jacquard, G. Wojnarowiez and C. Clement

2.4. Barley anther culture / L. Cistue, M. P. Valles, B. Echavarri, J. M. Sanz and A. Castillo

2.5. Anther culture for doubled haploid production in barley (Hordeum vulgare L.) / I. Szarejko

2.6. Barley isolated microspore culture protocol / K. J. Kasha, E. Simion, R. Oro and Y. S. Shim

2.7. Barley isolated microspore culture (IMC) method / P. A. Davies

2.8. Doubled haploid production in wheat through wide hybridization / M. N. Inagaki

2.9. Protocol of wheat (Triticum eastivum L.) anther culture / J. Pauk, R. Mihaly and M. Puolimatka

2.10. Protocol for producing doubled haploid plants from anther culture of wheat (Triticum aestivum L.) / B. Barnabas

2.11. Wheat anther culture / S. Tuvesson, R. von Post and A. Ljungberg

2.12. Haploid wheat isolated microspore culture protocol / K. J. Kasha, E. Simion, M. Miner, J. Letarte and T. C. Hu

2.13. Production of doubled haploids in wheat (Triticum aestivum L.) through microspore embryogenesis triggered by inducer chemicals / M. Y. Zheng, W. Liu, Y. Weng, E. Polle and C. F. Konzak

2.14. Isolated microspore culture in maize (Zea mays L.), production of doubled-haploid via induced androgenesis / M. Y. Zheng, Y. Weng, R. Sahibzada and C. F. Konzak

2.15. Anther culture of maize (Zea mays L.) / B. Barnabas

2.16. Laboratory protocol for anther culture technique in rice / F. J. Zapata-Arias

2.17. Triticale anther culture / S. Tuvesson, R. von Post and A. Ljungberg

2.18. Protocol for anther culture in hexaploid triticale (x Triticosecale Wittm) / M. Wedzony

2.19. Protocol of triticale (x Triticosecale Wittmack) microspore culture / J. Pauk, R. Mihaly, T. Monostori and M. Puolimatka

2.20. Protocol for doubled haploid production in hexaploid triticale (x Triticosecale Wittm.) by crosses with maize / M. Wedzony

2.21. Protocol for rye anther culture / S. Immonen and T. Tenhola-Roininen

2.22. Microspore culture of rye / S. Pulli and Y.-D. Guo

2.23. Oat haploids from wide hybridization / H. W. Rines

2.24. Haploid and doubled haploid production in durum wheat by wide hybridization / P. P. Jauhar

2.25. Haploid and doubled haploid production in durum wheat by anther culture / P. P. Jauhar

2.26. Anther culture and isolated microspore culture in timothy / S. Pulli and Y.-D. Guo

2.27. Doubled haploid induction in ryegrass and other grasses / S. B. Andersen

2.28. Microspore culture in rapeseed (Brassica napus L.) / J. B. M. Custers

2.29. Protocol for broccoli microspore culture / J. C. da Silva Dias

2.30. Microspore culture of Brassica species / A. Ferrie

2.31. Protocol for microspore culture in Brassica / M. Hansen

2.32. Anther and microspore culture in tobacco / A. Touraev and E. Heberle-Bors

2.33. Haploid production of potatoes by anther culture / G. C. C. Tai and X. Y. Xiong

2.34. Anther culture through direct embryogenesis in a genetically diverse range of potato (Solanum) species and their interspecific and intergeneric hybrids / V.-M. Rokka

2.35. Potato haploid technologies / M. J. De Maine

2.36. Anther culture of linseed (Linum usitatissimum L.) / K. Nichterlein

2.37. Doubled haploid production of sugar beet (Beta vulgaris L.) / E. Wremerth Weich and M. W. Levall

2.38. Asparagus microspore and anther culture / D. J. Wolyn and B. Nichols

2.39. In vitro gynogenesis induction and doubled haploid production in onion (Allium cepa L.) / L. Martinez

2.40. Haploid induction in onion via gynogenesis / M. Jakse and B. Bohanec

2.41. In vitro androgenesis in apple / M. Hofer

2.42. Doubled haploid production in poplar / S. B. Andersen

2.43. Oak anther culture / M. A. Bueno and J. A. Manzanera

2.44. Haploids and doubled haploids in Citrus spp / M. A. Germana

3. Published protocols for other crop plant species / M. Maluszynski, K. J. Kasha and I. Szarejko

4. Application of doubled haploid production techniques

4.1. Doubled haploids in breeding / W. T. B. Thomas, B. P. Forster and B. Gertsson

4.2. Doubled haploid mutant production / I. Szarejko

4.3. Barley microspore transformation protocol by biolistic gun / Y. S. Shim and K. J. Kasha

4.4. Doubled haploids in genetic mapping and genomics / B. P. Forster and W. T. B. Thomas

5. Protocols for chromosome counting

5.1. Cytogenetic tests for ploidy level analyses - chromosome counting / J. Maluszynska

5.2. Ploidy determination using flow cytometry / B. Bohanec

6. Major media composition and basic equipment for DH laboratory

6.1. Major media composition / I. Szarejko

6.2. Basic equipment for maize microspore culture laboratory / M. Y. Zheng, Y. Weng, R. Sahibzada and C. F. Konzak.